DHM in ImageJ

Digital Holographic Microscopy plugin for ImageJ. Allows realistic simulation and real-time reconstruction of single-shot holograms.

View the Project on GitHub unal-optodigital/DHM

Digital Holographic Microscopy (DHM)

An ImageJ Plugin

“DHM” is a plugin developed to work on the well-known software for image processing ImageJ or any of its derivate platforms like Fiji or Micro-Manager. This plugin allows both the realistic simulation and real-time reconstruction of digital holograms.

The simulation module implements a numerical telecentric image-plane DHM recording architecture with user-configurable imaging, interference, and scaling parameters. It also includes the possibility of defining an estimate of the average roughness of the sample to produce realistic coherent-noise affections.
Simulation GUI

The reconstruction module allows the spatial-filtering reconstruction of digital holograms and subsequent computation of either amplitude, intensity, or phase. The plugin receives as input either single images or video feeds for real-time processing. This module also implements user-defined fine-tuning parameters, allowing sub-pixel linear phase compensations and digital refocusing of the reconstructed fields.
Reconstruction GUI

Installation

The installation process is the standard procedure for any ImageJ plugin. Just download either the JAR or ZIP file and extract its contents under the imagej/plugins folder. It is advisable to use the latest release of ImageJ.

Once installed, you should be able to access the plugin at OD > DHM Fiji GUI Access

Downloads

You may choose any of the following:

References

Further information about this plugin and its functional modules can be found in the following publications. These are also the preferred way of citing this tool if you are implementing it in your works.

Contact

UNALODP